预染标准蛋白Marker（10-180kD） PPL1
SmartBuffers®Prestained Protein Ladder（10-180kDa）
预染标准蛋白Marker
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Cat. No. 目录编码 |
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PPL1 |
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Pack Size 包装规格 |
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250µL/Tube,100mL/Bottle,1L/Bottle |
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Appearance产品性状 |
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Blue-black Liquid |
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Storage Buffer 存储液 |
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62.5 mM Tris-H3PO4, pH7.5, 5mM EDTA, 2 % (W/V) SDS, 33 % (W/V) Glycerol, 0.02 % (V/V) proclin300 |
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Quality Control质控 |
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Tested in SDS-PAGE and Western Blotting |
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Shelf life效期 |
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Stable at -20°C for 24 months ; 4°C for 3 months; 25°C for 4 weeks |
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Description 产品描述
SmartBuffers®Prestained Protein Ladder is a ready-to-use formulation which does not require boiling, contains 10 prestained proteins covering a wide range of molecular weights (10~180 kDa) and includes a orange reference band at 70 kDa and a green reference band at 10 kDa. The protein ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western blot transfer (PVDF, nylon or nitrocellulose membranes) and for the approximate sizing of proteins. Supplied as a ready-to use formulation in gel loading buffer, the ladder requires no heating, dilution or addition of reducing agents.
Lot-to-lot variation of apparent molecular weight of prestained proteins is less than 3%.
Applications应用领域：
1) Monitoring of protein separation during SDS-PAGE.
2) Verifying Western transfer efficiency.
3) Approximate sizing of proteins on SDS-PAGE gels and Western blots.
4) Locating a protein of interest for excision from an unstained preparative gel.
Important Information 重要信息：
Ø Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.
Ø In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.
Ø SmartBuffers® Prestained Protein Ladder can be used in Western blotting with all common membranes: PVDF, nylon and nitrocellulose.
Ø Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.
Ø the proteins in the ladder have his-tag,when use anti-histag western bloting
Preparation使用说明：
1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil!
2. Mix gently, but thoroughly, to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on an SDS-polyacrylamide gel:
- 5 μL per well for mini gel,
-10 μL per well for large gel.
4. Use the same volumes for Western blotting.
5. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.
Migration patterns of SmartBuffers® Prestained Protein Ladder：
The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis.
