PPL3 预染标准蛋白Marker(10-130kDa)

Description 产品描述

SmartBuffers^®Prestained Protein Ladder(Cat No.:PPL3) is a ready-to-use formulation which does not require boiling, contains 9 prestained proteins covering a wide range of molecular weights (10~130 kDa) and includes a orange reference band at 70 kDa and a green reference band at 10 kDa. The protein ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western blot transfer (PVDF, Nylon or Nitrocellulose membranes) and for the approximate sizing of proteins. Supplied as a ready-to use formulation in gel loading buffer, the ladder requires no heating, dilution or addition of reducing agents.

Lot-to-lot variation of apparent molecular weight of prestained proteins is less than 3%.

Important Information 重要信息：

Prestained proteins can have different mobilities in various SDS-PAGE-buffer systems. However, they are suitable for approximate molecular weight determination when calibrated against unstained standards in the same system. See the table provided for migration patterns in different electrophoresis conditions.

In low-percentage gels (< 10 %), the low-molecular weight proteins in the ladder may migrate with the dye front.

SmartBuffers^® Prestained Protein Ladder can be used in Western Blotting with all common membranes: PVDF, Nylon and Nitrocellulose.

Longer transfer times or higher transfer voltages may be required for Western blotting of large (>100 kDa) proteins.

Preparation使用说明：

1. Thaw the ladder at room temperature for a few minutes to dissolve precipitated solids. Do not boil !!

2. Mix gently, but thoroughly, to ensure the solution is homogeneous.

3. Load the following volumes of the ladder on an SDS-polyacrylamide gel:

- 5 μL per well for mini gel,

-10 μL per well for large gel.

4. Use the same volumes for Western blotting.

5. The loading volumes listed above are recommended for gels with a thickness of 0.75-1.0 mm. The loading volume should be doubled for 1.5 mm thick gels.

Migration patterns of SmartBuffers^® Prestained Protein Ladder：

The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis.

                                   

 

                                                  

                                                      

                                                       Gel  12.5% Tris-Glycine  Blot